C-Dish and C-Pace EM Chronic Cell Stimulation
Key Features
c-dish and the C-Pace EM chronic cell stimulation system provide a complete solution for chronic electrical stimulation of bulk quantities of excitable cells in culture. The system consists of a dish with carbon electrode elements and a power supply for field stimulator connection.
The CPace EM has been engineered for optimal use with the c-dish. It is used to stimulate excitable cells in a tissue culture dish and can be connected to the carbon electrodes of the c-dish by a simple ribbon cable.
In addition to the known pattern of illumination of the primary mirror, the CPace EM is capable of generating other dish illumination patterns for specific performance requirements. These include patterns with high taper at the dish edge for low spillover sidelobes and patterns with a central “hole” to reduce feed shadowing. The system also can generate a bespoke pattern for the secondary reflector to further improve performance. This involves a complex geometrical manipulation of the secondary surface to achieve a very precise hyperbolic shape.
Specifications
Older mesh BUDs have large perforations and are inefficient at Ku frequencies, while new systems use a steel dish with a smaller reflector, and can include an LNB that is electronically switched between horizontal and vertical polarization, obviating the need for a failure-prone polar rotator. The stock BUD LNB typically needs to be replaced with one capable of receiving FTA or DBS digital Ku signals, and should have a lower noise temperature.
Works with the C-Pace EM chronic cell stimulator to provide a complete system for the chronic stimulation of bulk quantities of cardiac myocytes in culture.
Applications
The patented C-Dish works with the C-Pace EM chronic cell stimulator to allow stimulation experiments of bulk quantities of excitable cells in standard culture plates. It consists of a simple 2-piece assembly that precisely places carbon electrode elements in standard culture plates and connects them to the stimulator through pin connectors.
The low walls of ibidi u-Dishes (particularly the u-Dish 35 mm, low polymer bottom) make them ideal for micromanipulation applications such as intracellular injection or micropipette chemotaxis assays. They can also be used for tissue treatment and high-resolution live cell imaging.
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